Employing marine invertebrate cell culture media for isolation and cultivation of thraustochytrids

Abstract Thraustochytrids, a common group of marine eukaryotic protists, have drawn considerable scientific and industrial interest due to their ability synthesize high levels bioactive compounds, including polyunsaturated fatty acids, docosahexaenoic acid, squalene carotenoids, new applications for biofuels. The pharmaceutical potential thraustochytrids necessitate effective isolation strains establishment axenic cultures. To date, existing protocols used baiting direct plating methods generate cultures with varied media compositions that contain peptone yeast extracts as nitrogen sources, glucose carbon source, seawater antibiotics. Here we reveal approach the from tissues invertebrates using (a) primary cell in liquid medium containing basal medium, 50% artificial seawater, vitamins, proteins antibiotics, (b) cultivation same culture medium. Using colonial tunicate Botryllus schlosseri model system, thraustochytrid cells thrived day extraction, grew proliferated next five weeks (five-passages, up 1.9 × 10 6 ml −1 passage 5; 1.45-fold multiplication week ). This enables species promising compounds.